Table 3 - T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics

Table 3. Functionally characterized genes from Arabidopsis using T-DNA.

a. Forward genetic approach.

S. No.	Genes	Functions	References
1	GL1	Trichome development	Marks and Feldmann, 1989
2	CPR-20	Disease resistance signalling	Silva et al. 1999
3	GI	Phytochrome signalling	Huq et al. 2000
4	MYB75	Anthocyanin production	Borevitz et al. 2000
5	LEP	Leaf development	Van der Graaff et al. 2000
6	DPE1	Starch breakdown	Critchley et al. 2001
7	Dfl1	Auxin control of root development	Nakazawa et al. 2001
8	KAN	Organ polarity	Kerstetter et al. 2001
9	BRS1	Brassinosteroid signaling	Li et al. 2001
10	QUA1	Biosynthesis of pectins	Bouton et al.2002
11	ATE1	Leaf senescence	Yoshida et al. 2002
12	PRPS17	Leaf senescence	Woo et al. 2002
13	PLS	Auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning	Casson et al. 2002
14	CPL1 and CPL3	Abiotic stress signalling growth and development	Koiwa et al. 2002
15	OSM1 and SYP61	Stress tolerance and ABA regulation of stomatal response	Zhu et al. 2002
16	HCF152	RNA processing	Meierhoff et al. 2003
17	EXO	Cell division	Farrar et al. 2003
18	OMT1	Lignin and synapoyl ester biosynthesis	Goujon et al. 2003b
19	ULI3	UVB light response	Suesslin and Frohnmeyer, 2003
20	TPT	Acclimation of photosynthesis to environment	Walters et al. 2003
21	AMS	Tapetal and Microspore development	Sorensen et al. 2003
22	BXL1	cell wall metabolism	Goujon et al. 2003a
23	Cpn60 beta	Molecular chaperonin	Ishikawa et al. 2003

b. Reverse genetic approach.

S. No.	Genes	Functions	References
1	PGP1	Photosynthesis	Hagio et al. 2002
2	SEC and SPY	Gametogenesis and embryogenesis	Hartweck et al.2002
3	PGP1	Photosynthesis	Hagio et al. 2002
4	AMT1;1	NH4+transporter	Kaiser et al. 2002
5	LACS9	Chloroplast Acyl-coenzyme A (CoA) synthetase	Schnurr et al. 2002
6	DIR1	systemic resistance signalling	Maldonado et al. 2002
7	GEM1-1 and GEM1-2,	cytokinesis	Twell et al. 2002
8	ECA1	Ca²⁺ pump	Wu et al. 2002
9	MRE11 and KU70	Telomere maintenance and DNA repair	Bundock and Hooykaas, 2002
10	IRT1	Metal transporter	Henrique et al. 2002
11	AtTOP6B	DNA replication	Hartung et al. 2002
12	MMT	Selenium volatalisation	Tagmount et al. 2002
13	RCN1	ABA signal transduction	Kwak et al. 2002
14	CKI1	Megagametophyte development	Pischke et al. 2002
15	MMP	Flowering and early senescence	Golldack et al. 2002
16	AtOPT3	Embryo development	Stacey et al. 2002
17	AtpOMT1, AtpDCT1, AtpDCT2	Oxoglutarate/Malate and Dicarboxylate Transporters	Taniguchi et al. 2002
18	PEX10	Peroxisome biogenesis	Schumann et al. 2003
19	UVH6	DNA repair (UV resistance) and growth	Liu et al. 2003
20	UGT73C6 and UGT78D1	Flavonol glycoside biosynthesis	Jones et al. 2003
21	CAP-E1	Embryogenesis, meosis and Meristem organization	Siddiqui et al. 2003
22	LUT1	Carotenoid hydroxylase	Tian et al. 2003
23	SCD1 and Arp2/3	Polar cell expansion	Falbel et al. 2003; Li et al. 2003
24	CLAVATA1	Organ development	Dievart et al.2003
25	ATHB5	Abscisic acid signal transduction	Johannesson et al. 2003
26	TDS4	Anthocyanidin synthesis	Abrahams et al. 2003
27	AtFUT1	Xyloglucan fucosilation (cell wall biosynthesis)	Perrin et al. 2003
28	ARR15	Cytokinin mediated signal transduction	Kiba et al. 2003
29	ATM	Meiosis and somatic response to DNA damage	Garcia et al. 2003
30	MAF2	Vernalisation	Ratcliffe et al. 2003
31	Apyrases (Atapy1, Atapy2)	Pollen germination	Steinebrunner et al. 2003
32	GORK	Stomatal movements and transpiration	Hosy et al. 2003
33	FATB	Fatty acid biosynthesis	Bonaventure et al. 2003
34	GGAT	Photorespiration	Igarashi et al. 2003
35	ACT7	Germination and root growth	Gilliland et al.2003
36	PIP2;2	Hydraulic conductivity of roots	Javot et al. 2003
37	ADA2b and GCN5	Growth, development, and gene expression	Vlachonasios et al. 2003

23

SCD1 and Arp2/3