Table 1 - An improved system for competent cell preparation and high efficiency plasmid transformation using different Escherichia coli strains

Table 1. Transformation efficiency of plasmid DNA to different E. coli strains (Average).

XL1 blue E. coli strains

DH5a E. coli strains

TG1 E. coli strains

plasmid

Normal method (Sambrook, 1989)

Quick method(Chen, 2001)

This improved method

Normal method (Sambrook 1989)

Quick method(Chen, 2001)

This improved method

Normal method (Sambrook 1989)

Quick method(Chen, 2001)

This improved method

pUC18

6.51x10⁶

7.1x10⁵

8.18x10⁸

2.31x10⁶

3.5x10⁵

4.15x10⁸

2.01x10⁶

3.8x10⁵

4.5x10⁸

pUGFP

7.79x10⁶

6.7 x10⁵

8.85x10⁸

1.66x10⁶

3.2 x10⁵

2.63x10⁸

1.25x10⁶

2.2 x10⁵

4.72x10⁸

pAHC25

6.86x10⁶

7.8 x10⁵

9.09x10⁸

1.56x10⁶

2.7 x10⁵

4.13x10⁸

1.46x10⁶

2.9 x10⁵

5.39x10⁸

p1Ax1

7.13x10⁶

6.4 x10⁵

8.6 x10⁸

1.25x10⁶

2.3x10⁵

4.52x10⁸

1.08x10⁶

2.7 x10⁵

4.26x10⁸

p1Dx5

7.09x10⁶

6.98x10⁵

8.9 x10⁸

1.31x10⁶

2.08x10⁵

3.99x10⁸

1.21x10⁶

2.08x10⁵

4.19x10⁸

p1Dy10

8.17x10⁶

6.75x10⁵

7.8x10⁸

1.20x10⁶

1.97x10⁵

3.56x10⁸

1.31x10⁶

1.97x10⁵

3.25x10⁸