| Electronic Journal of Biotechnology
ISSN: 0717-3458 |
Vol. 8 No. 2, Issue of August 15, 2005 |
| © 2005 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received February
8, 2005 / Accepted May 4, 2005 |
Expression of the antibody 14D9
in Nicotiana tabacum hairy roots
Carolina Martínez
Cátedra
de Microbiología Industrial y Biotecnología
Facultad de Farmacia y Bioquímica
Universidad de Buenos Aires
Buenos Aires, Argentina
Junín 956 6 piso 1113
Tel: 54 11 4964 8269
Fax: 54 11 4964 8200 ext.8377
E-mail: carom@ffyb.uba.ar
Silvana
Petruccelli
CIDCA
Facultad de Ciencias Exactas
Universidad Nacional de la Plata
Argentina
Calle 47 y 116 1900
La Plata, Argentina
Tel: 54 221 4249287
Fax : 54 221 4254853
E-mail: silvana@biol.unlp.edu.ar
Ana
María Giulietti
Cátedra de Microbiología Industrial y Biotecnología
Facultad de Farmacia y Bioquímica
Universidad de Buenos Aires
Buenos Aires, Argentina
Junín 956 6 piso 1113
Tel: 54 11 4964 8269
Fax: 54 11 4964 8200 ext.8377
E-mail: agiule@ffyb.uba.ar
María
Alejandra Alvarez*#
Cátedra de Microbiología Industrial
y Biotecnología
Facultad de Farmacia y Bioquímica
Universidad de Buenos Aires
Buenos Aires, Argentina
Junín 956 6 piso 1113
Tel: 54 11 4964 8269
Fax: 54 11 4964 8200 ext. 8377
E-mail: malvarez@ffyb.uba.ar
*Corresponding
author
Financial
support: This research was supported by CONICET and the University
of Buenos Aires.
Keywords:
catalytic antibodies, in vitro cultures, molecular farming,
recombinant antibodies, tobacco.
Present address:
#Fundación
Pablo Cassará, Saladillo 2452, Ciudad de Buenos Aires, (C1144OFFX),
Tel: 54 11 4886 5242, Fax: 54 11 4065.
| Abbreviations: |
DMSO:
dimethylsulfoxide;
ER: endoplasmic
reticulum;
FW: fresh
weight;
GI: growth
index = (Final FW- Inicial FW)/ Initial FW;
PVP: polyvinylpyrrolidone;
TSP: total
soluble protein.
|
| |
Nicotiana tabacum
hairy roots that express the antibody 14D9 were established. The 14D9
antibody yield obtained after 20 days of culture was 5.95 μg
14D9ml-1. The addition of the reticulum endoplasmic retention
sequence KDEL demonstrated a positive effect over the intracellular
14D9 amounts with a yield increase up to 20.82 µg ml-1.
DMSO increased the antibody amount in the biomass from 20.00 to 64.03
µg ml-1 while PVP (at 1.5 gl-1) and gelatine
(at 5.0 gl-1) increased total 14D9 amounts in the culture
medium to 25 µg and 14 µg respectively.
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