Microbial Biotechnology

Biotechnology Industry

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 8 No. 2, Issue of August 15, 2005
© 2005 by Pontificia Universidad Católica de Valparaíso -- Chile Received July 20, 2004 / Accepted April 8, 2005
RESEARCH ARTICLE

Potential application of protease isolated from Pseudomonas aeruginosa PD100

Mohsen Fathi Najafi*
Razi Institute
Next to Hotel Homa, Ahmadabad street
Mashad , Iran
Tel: 98 511 8431780/8418114
Fax: 98 511 8420430
E-mail: najafi99@yahoo.com

Dileep Deobagkar
The Molecular Biology Research Laboratory
Department of Zoology
University of Pune
Ganeshkhind, Pune-411007, Maharashtra, India
Tel: 91 20 52698432
Fax:91 20 52690617
E-mail: dddeo@unipune.ernet.in

Deepti Deobagkar
The Molecular Biology Research Laboratory
Department of Zoology
University of Pune
Ganeshkhind, Pune-411007, Maharashtra, India
Tel: 91 20 52698432
Fax:91 20 52690617
E-mail: dddeo@unipune.ernet.in

*Corresponding author


Financial support: Government of India and Iran

Keywords: blood clot, protease, Pseudomonas aeruginosa PD100, SDS, solvent.

Abstract
Full Text

A protease isolated from Pseudomonas aeruginosa PD100 could act in the presence of SDS and Tween 80. This protease could be useful for degradation of protein in the presence of solvent, dehairing of cow skin and degradation of natural proteins. The immobilized protease showed 15-20% increases in temperature stability and the entrapped enzyme retained 83% of its initial activity after six cycles. With respect to properties of the enzyme and its capability for degradation of different protein sources, this protease finds potential application for waste treatment, used in detergents and leather industry.

 

 
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