Figure 5. Western-blot of hairy root biomass. The lines were loaded with equal volumes of samples. Line 1: Standard IgG Antibody; line 2: N. tabacum wild type; line 3: N. tabacum wild type + 5% DMSO; line 4: Ac₂ + 2.8% DMSO; line 5: Ac₂ + 5% DMSO; line 6: Ac₂ control; line 7: AcK₆ + 2.8% DMSO; line 8: AcK₆+5%; line 9: AcK₆ control. Coating: gaot anti gamma mouse chain conjugated with peroxidase (1:1000) in 1% (P/V) non fat milk. Antibody binding was detected after incubation with Supersignal West Pico Chemiluminiscent Substrate (Pierce Chem.) after 25 min of exposure.