| Figure 1. Diagrammatic representation of the bioreactor model used for the growth of hairy root clone LMG-150. The reactor was of 3 L capacity with a working volume of 1.75 L. About 10 g of actively growing hairy roots were inoculated in to the anchorage basket held in growth vessel having MS basal liquid medium with 3% sucrose. The air was supplied at a rate of 33.4 cm3 s-1 through the glass sparger of 45 cm height and 7 cm diameter. The reactor was operated in dark at 23 ± 2ºC and after the required running period the reactor was dismantled and the biomass and spent medium were analyzed for POD. |
