Figure 3. Pattern of growth and biomass accumulation in different clones of hairy roots over a 25-day growth period. About 50 mg of root tips each of the 10 hairy root clones were subcultured in 50 ml Erlenmeyer's flasks containing 15 ml of MS basal liquid medium with 3% sucrose and grown on a rotary shaker set at 90 rpm in dark at 23 ± 2ºC. The biomass accumulation was monitored at an interval of 5 days for a total period of 25 days. Fresh weight increase was recorded after surface drying the roots by keeping between folds of blotter sheets. The data is the average of 5 replicates of two independent experiments each and the bars indicate SE of mean.