Figure 7. Zymogram of peroxidase isozymes from roots (Lanes 1 - 4) and from spent medium (lanes 5 - 8) developed by activity staining of the enzyme separated on 7.2% native polyacrylamide gel. The electrophoresis was carried out by loading about 20 μl each of crude enzyme preparation from both biomass and spent medium in a 7.2 % polyacrylamide gel (12 ´ 14 ´ 0.3 cm) (PAGE) carried out at 120V for 4 hrs. Similarly about 10 μl of standard protein molecular weight marker mix was also loaded and stained with coomassie brilliant blue R-250 (lane M) separately after electrophoresis.