Table 3 - Wild ornamental germplasm exploration and domestication based on biotechnological approaches. In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis

Table 3. Number of shoots per explant and tetraploids or chimeric plants obtained through colchicine treatment in Scoparia montevidiensis.

Treatment		No. of shoots per explants (mean ± SD)^z	No. of plants analyzed	No. of plants
Conc. of Colchicine (%)	Duration (hours)	No. of shoots per explants (mean ± SD)^z	No. of plants analyzed	2X/4X	4X
Control 1 ^y	---	11.16 ± 1.16 a	5	0	0
Control 2 ^x	24	12.12 ± 1.80 a	10	0	0
Control 2'^x	48	12.20 ± 1.70 a	5	0	0
Control 3 ^w	24	11.00 ± 1.41 a	5	0	0
Control 3' ^w	48	11.17 ± 1.16 a	5	0	0
0.001	24	11.00 ± 2.44 a	50	3	2
0.001	48	12.50 ± 2.07 a	24	1	1
0.01	24	9.57 ± 1.71 a	35	1	0
0.01	48	10.71 ± 1.97 a	40	1	0
0.05	24	9.00 ± 3.34 a	60	6	1
0.05	48	9.87 ± 2.79 a	40	2	0
0.1	24	6.85 ± 2.41 b	50	1	0
0.1	48	6.57 ± 1.51 b	70	0	0
Total			379	15	4

^z Same letter indicates no significant differences.
^y Untreated plants.
^x Water treatment
^w DMSO treatment.