Figure 1. Effect of SDS and Tween 80 on protease. The enzyme was incubated with 1% (w/v) SDS and 3% (v/v) Tween 80 along with 1.5% (w/v) casein in 50 mM Tris-HCl (pH 8) at 50ºC . Aliquots were removed at different time intervals and the amount of tyrosine released was determined according to standard method.