Figure 2. Influence of different solvents on activity of the protease. The enzyme was assayed in the presence of different amount of solvent, [10%, 20% and 50% (v/v) of each solvent] for 20 min at 55ºC. The residual activities were determined with respect to controls. Methanol (M), 1-propanol (1-P), 2-propanol (2-P), ethyleneglycol (E), ethylacetate (Et), acetone (A), xylene (X), toluene (T), benzene (B).