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Table 2. Properties of the protease from Pseudomonas aeruginosa PD100. |
Property |
Value |
Molecular weight by: |
|
Number of protease |
One protease was secreted by the organism (confirmed by Acidic-Native-PAGE and Native-PAGE) |
Carbohydrate content |
NIL |
pI |
6.2 |
Optimum pH |
8 |
pH stability |
pH 6.5-11 for 24 h at 4ºC |
Optimum temperature |
60ºC |
Temperature stability |
55ºC for 60 min |
Metal ion requirement |
NIL |
Inhibitors |
Ag2+, Ni2+, Cu2+, Zn2+, β-ME, DTNB, iodoacetamide, DEPC, HNBB, NEM |
Mode of action |
Endo |
Substrate specificity |
collagen> fibrin> azocasein> casein> hemoglobin> BSA> ovalbumin> elastin |
Auto digestion |
NIL |
|
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