|
|
Table 1. Purification
of chitinase by ammonium sulphate, DEAE-Sephadex column chromatography
and Sephadex G-200 gel filtration. |
| Purification Step |
Total
Activity |
Total
protein |
Specific
activity |
Purification fold |
Recovery |
| Cell free supernatant |
45100 |
255 |
176.4 |
- |
100 |
| Dialysed (ammonium sulphate precipitation 30-75%) |
31950 |
57 |
560.5 |
3.18 |
71.0 |
| DEAE Sephadex |
22500 |
5.5 |
4090.9 |
23.2 |
47.1 |
| Gel
filtration |
14010 |
1.8 |
7783.3 |
44.12 |
31.1 |
|
|