Table 3. Relative Synonymous Codon Usage values (RSCU) in A. hypogaea, L. japonicus, A. thaliana, G. max and M. truncatula. Values given are RSCU (relative synonymous codon usage).

Amino acid Name	Codon triplet	Arachis hypogaea	Lotus japonicus	Arabidopsis thaliana	Glycine max	Medicago truncatula
Ala	GCA	1.3*	0.8	1.0	1.3	1.4
	GCC	1.0	1.2	0.6	0.8	0.5
	GCG	0.3	0.3	0.5	0.4	0.2
	GCT	1.3*	1.5*	1.7*	1.5*	1.8*
Cys	TGC	1.2*	1.0*	0.8	0.8	0.6
	TGT	0.7	0.9*	1.2*	1.2*	1.3*
Asp	GAC	0.8	0.9*	0.6	0.7	0.5
	GAT	1.1*	1.0*	1.4*	1.3*	1.4*
Glu	GAA	0.9*	0.9*	1.1*	1.3*	1.1*
	GAG	1.0*	1.0*	0.9	0.7	0.8
Phe	TTC	1.2*	1.2*	0.9*	0.6	0.8
	TTT	0.7	0.7	1*	1.4*	1.1*
Gly	GGA	1.3*	1.1*	1.5*	1.4*	1.5*
	GGC	0.9	1.1*	0.5	0.7	0.4
	GGG	0.5	0.5	0.6	0.8	0.4
	GGT	1.1	1.1*	1.4	1.1	1.5*
His	CAC	1.0*	1.1*	0.8	0.7	0.6
	CAT	0.9*	0.8	1.2*	1.3*	1.3*
Val	GTA	0.4	0.2	0.6	0.9	0.6
	GTC	0.7	1.0	0.8	0.6	0.5
	GTG	1.2	1.1	1.0	0.9	0.9
	GTT	1.6*	1.5*	1.6*	1.5*	1.9*
Ile	ATA	0.6	0.4	0.7	1.0	0.8
	ATC	1.2*	1.4*	1.0	0.6	0.7
	ATT	1.1*	1.1	1.2*	1.3*	1.4*
Lys	AAA	0.7	0.9	0.9	0.6	1.0*
	AAG	1.2*	1.1*	1*	1.3*	0.9*
Leu	CTA	0.4	0.4	0.6	0.7	0.6
	CTC	1.4	1.7*	1.0	0.7	0.6
	CTG	0.6	0.7	0.6	0.5	0.4
	CTT	1.7*	1.4	1.5*	1.4*	1.7*
	TTA	0.4	0.3	0.8	1.2	0.9
	TTG	1.3	1.1	1.3	1.3	1.5
Asn	AAC	1.3*	1.2*	0.9*	1.3*	0.7
	AAT	0.6	0.7	1.0*	0.6	1.2*
Pro	CCA	1.4*	1.0	1.3	1.4*	1.6*
	CCC	0.6	1.0	0.4	0.8	0.4
	CCG	0.5	0.6	0.7	0.4	0.3
	CCT	1.3	1.3*	1.5*	1.4*	1.6*
Trp	TGG	1	1	1	1	1
Gln	CAA	1.0*	1.0*	1.1*	1.4*	1.3*
	CAG	0.9*	0.9*	0.8	0.6	0.6
Arg	AGA	1.6*	1.4*	2.1*	2.1*	2.2*
	AGG	1.7*	1.4*	1.2	1.5	1.4
	CGA	0.4	0.7	0.7	0.7	0.5
	CGC	0.8	1.1	0.4	0.5	0.4
	CGG	0.4	0.4	0.5	0.5	0.3
	CGT	0.8	0.8	1.0	0.7	1.0
Ser	AGC	1.3*	0.8	0.7	0.7	0.6
	AGT	0.8	0.5	0.9	1.0	1.0
	TCA	1.2*	1.2	1.2	1.3	1.6*
	TCC	1.0	1.5*	0.7	0.9	0.7
	TCG	0.4	0.3	0.6	0.4	0.3
	TCT	1.1	1.5*	1.7*	1.4*	1.6*
Thr	ACA	1.0	1.0	1.2	1.2	1.5*
	ACC	1.3*	1.5*	0.8	0.8	0.7
	ACG	0.3	0.4	0.6	0.4	0.2
	ACT	1.2	0.9	1.4*	1.4*	1.5*
Tyr	TAC	1.0*	1.2*	0.9*	0.6	0.8
	TAT	0.9	0.7	1.0*	1.4*	1.1*
TER	TAG	0.7	0	0.6	0.7	0.6
	TGA	0.9	0	1.3	0.9	1.2
	TAA	1.3	0	1.0	1.3	1.1
Total number of codons		19531	9071	25973879	74581	101743

*most frequently used codons