Abstract - A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

Maryam Sarwat
Department of Biotechnology
Jamia Hamdard (Hamdard University)
New Delhi 110062, India
Tel: 091 11 26059688
Fax: 091 11 26059663
E-mail: maryam21_7@yahoo.com

Madan Singh Negi
TERI, India Habitat Center
Lodhi Road, New Delhi 110003, India
Tel: 091 11 24682111
Fax: 091 11 24682144
E-mail: msnegi@teri.res.in

Malathi Lakshmikumaran
TERI, India Habitat Center
Lodhi Road, New Delhi 110003, India
Tel: 091 11 26192243
Fax: 091 11 26197578
E-mail: malathi@lakshmisri.com

Akhilesh Kumar Tyagi
Department of Plant Molecular Biology
University of Delhi South Campus
Benito Juarez Marg, New Delhi 110021, India
Tel: 091 11 24113216
Fax: 091 11 24119430
E-mail: akhilesh@genomeindia.org

Sandip Das
Department of Biotechnology
Jamia Hamdard (Hamdard University)
New Delhi 110062, India
Tel: 091 11 26059688
Fax: 091 11 26059663
E-mail: sdas@jamiahamdard.ac.in

Prem Shankar Srivastava*
Department of Biotechnology
Jamia Hamdard (Hamdard University)
New Delhi 110062, India
Tel: 0091 11 26059688 ext 5582
Fax: 0091 11 26059663
E-mail: pss410@rediffmail.com

*Corresponding Author

Financial support: Department of Biotechnology, Govt. of India.

Keywords: DNA extraction, medicinal plants, molecular markers, Terminalia species.

For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).