Microbial Biotechnology

Biotechnology of Human Disorders

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 1, Issue of January 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile Received April 18, 2005 / Accepted August 10, 2005
DOI: 10.2225/vol9-issue1-fulltext-7  
RESEARCH ARTICLE

A new method: measurement of microleakage volume using human, dog and bovine permanent teeth

Izzet Yavuz*
Faculty of Dentistry
Department of Pediatrics
University of Dicle
21280, Diyarbakir, Turkey
Tel: 90 412 2488101/3426
Fax: 90 412 2488100
E-mail: iyavuz@dicle.edu.tr

Haluk Aydin
Chemistry Department
Faculty of Sciences
Dicle University
21280 Diyarbakır, Turkey
Tel: 90 412 2488550 1 etx. 3164

Refik Ulku
Department of Thoracic Surgery
Faculty of Medicine
Dicle University
21280, Diyarbakir, Turkey
Tel: 90 412 2488001 16 ext. 4263

Sadullah Kaya
Department of Operative Dentistry
Faculty of Dentistry
Dicle University
21280, Diyarbakir, Turkey
Tel: 90 412 2488101 ext. 3423
Fax: 90 412 248 8100

Caner Tumen
Faculty of Dentistry
Department of Pediatrics
University of Dicle
21280, Diyarbakir, Turkey
Tel: 90 412 2488101 ext. 3406
Fax: 90 412 248 8100

*Corresponding author

Keywords: dye adsorption test, microleakage, tooth.

Abbreviations:

methylene blue (MB)

Abstract Reprint (PDF)

This study investigates the feasibility of a different new approach to determining the microleakage volume associated with dental restorations (Class V cavity restorated with glass ionomer cement + high copper amalgam) and the relative marginal adaptation deficiency of dog, bovine and human permanent teeth in in vitro conditions. Also researched is the appropriateness of using dog and bovine teeth in in vitro studies rather than human teeth. Our method utilizes the molecular adsorption characteristics of methylene blue. Within the framework of this study, 60 permanent teeth (20 human, 20 dogs and 20 bovine) were used. These groups were evaluated statistically, of which indicated no statistically significant differences (p > 0.05). It was also concluded that this preliminary investigation showed that the new microleakage volume measurement method may be a valuable new technique for the in vitro study of microleakage dynamics around dental restorations.

 
Supported by UNESCO / MIRCEN network 
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