Table 1. Amount of water (% (v/v)) added to the organic solvents necessary to obtain a constant relative fraction of water in the enzyme, independently of the hydrophobicity of the used solvent.

Organic solvent

% (v/v) of water added to the organic solvent

% (w/w) of water in the enzyme

Relative fraction of water in the enzyme

Acetonitrile

5.6

50

0.5

Acetophenone

2.13

49.8

0.5

Chlorobenzene

2.5

50.26

0.5

Ciclohexane

0.89

50.08

0.5

Diethyl ether

1.02

50.22

0.5

Dichloromethane

3.4

50

0.5

Ethanol

9.43

50

0.5

Ethyl acetate

2.5

50

0.5

Ethylene glycol

17.8

50

0.5

Methanol

13

50

0.5

N,N-dimethylformamide

18.57

50

0.5

1-Octanol

0.63

50

0.5

Propanone

7.25

50

0.5

Tetrahydrofuran

5

50

0.5

Toluene

0.88

50.28

0.5

Trichloroethylene

2

50.4

0.5


Supported by UNESCO / MIRCEN network