Table 1. Amount of water (% (v/v)) added to the organic solvents necessary to obtain a constant relative fraction of water in the enzyme, independently of the hydrophobicity of the used solvent. |
Organic solvent |
% (v/v) of water added to the organic solvent |
% (w/w) of water in the enzyme |
Relative fraction of water in the enzyme |
Acetonitrile |
5.6 |
50 |
0.5 |
Acetophenone |
2.13 |
49.8 |
0.5 |
Chlorobenzene |
2.5 |
50.26 |
0.5 |
Ciclohexane |
0.89 |
50.08 |
0.5 |
Diethyl ether |
1.02 |
50.22 |
0.5 |
Dichloromethane |
3.4 |
50 |
0.5 |
Ethanol |
9.43 |
50 |
0.5 |
Ethyl acetate |
2.5 |
50 |
0.5 |
Ethylene glycol |
17.8 |
50 |
0.5 |
Methanol |
13 |
50 |
0.5 |
N,N-dimethylformamide |
18.57 |
50 |
0.5 |
1-Octanol |
0.63 |
50 |
0.5 |
Propanone |
7.25 |
50 |
0.5 |
Tetrahydrofuran |
5 |
50 |
0.5 |
Toluene |
0.88 |
50.28 |
0.5 |
Trichloroethylene |
2 |
50.4 |
0.5 |
|