Table 4. Culture parameters of CHO cell cultures: Effect of specific amino acids supplementation.

 

 

C7

C8

Parameter

CC

% control

µ (h-1)

0.0180 ± 0.0019

159

162

Xv (106 cel ml-1)a

0.72 ± 0.04

230

251

CV (%)a

76.0 ± 4.0

130

139

qt-PA (ng 10-6 cells h-1)

21.8 ± 2.0

87

98

t-PA (mg l-1)a 

1.01 ± 0.06

142

158

 at-PA(U mg-1)a

102.0 ± 7.1

136

134

qGlc  (nmol 10-6 cells h-1)

181.0 ± 5.4

95

94

qLac(nmol 10-6 cells h-1)

362 ± 14

89

92

qGln(nmol 10-6 cells h-1)

87.0 ± 4.5

83

80

qAmm(nmol 10-6 cells h-1)

130.0 ± 8.7

70

94


a: 146 hrs of culture.
Supported by UNESCO / MIRCEN network