Environmental Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 2, Issue of April 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 6, 2005 / Accepted October 28 , 2005
DOI: 10.2225/vol9-issue2-fulltext-10  
RESEARCH ARTICLE

Biotransformation of anthracene and fluoranthene by Absidia fusca Linnemann

Danièle Villemain
Laboratoire Radiopharmaceutiques Biocliniques
INSERM 0340, UFR de Médecine de Grenoble
Université Joseph Fourier
38706 La Tronche cedex, France
Tel: 33 4 76637100
E-mail: Daniele.Villemain@ujf-grenoble.fr 

Pascale Guiraud*
Laboratoire Oligoéléments et Résistance au Stress Oxydant induit par les Xénobiotiques
ORSOX, EA3776 - CEA LRC 8M, UFR de Pharmacie de Grenoble
Université Joseph Fourier
38706 La Tronche cedex, France
Tel: 33 4 76637515
Fax: 33 4 76637423
E-mail: Pascale.Guiraud@ujf-grenoble.fr

Ouahiba Bordjiba
Institut des Sciences de la Nature
Université d'Annaba
23000 Annaba, Algeria
Tel: 213 38875400
Fax: 213 38875400
E-mail: ouahiba_bordjiba@yahoo.fr 

Régine Steiman
Laboratoire Oligoéléments et Résistance au Stress Oxydant induit par les Xénobiotiques
ORSOX, EA3776 - CEA LRC 8M, UFR de Pharmacie de Grenoble
Université Joseph Fourier
38706 La Tronche cedex, France
Tel: 33 4 76637515
Fax: 33 4 76637423
E-mail: Regine.Steiman@ujf-grenoble.fr

*Corresponding author

Keywords: Absidia fusca, anthracene, biodegradation, fluoranthene, kinetics.

Abbreviations:

AC: anthracene
CV: coefficient of variation
FA: fluoranthene
GS: Galzy Slonimski (liquid medium)
HPLC: High Performance Liquid Chromatography
MEA: malt extract agar
PAHs: polycyclic aromatic hydrocarbons
PLSD: Protected Least Significant Difference
S: strain
T: temperature
t: time
Abstract Reprint (PDF)

A strain of Absidia fusca was isolated from a pesticide-contaminated soil (Annaba, Algeria). The biotransformation capability of this strain towards two polycyclic aromatic hydrocarbons (PAHs): anthracene and fluoranthene was compared to that exhibited by another strain of A. fusca isolated from a non-contaminated milieu and considered as a control. The results obtained were statistically analyzed and showed that the strain isolated from the contaminated soil was more efficient than the control to remove anthracene from the medium, during all the kinetics (90% removed versus 45% after 24 hrs). Concerning fluoranthene, the amount removed by both strains was very high during the first 24 hrs however the control strain was slightly more efficient (94% versus 89%) while the results were similar for the two strains during the rest of the kinetics. This study reveals for the first time the potential interest of the species A. fusca for the bioremediation of PAHs.

 
 
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