Figure 2. Validity of PAb-MnSOD in detection of different susceptible and resistant pearl millet genotypes after inoculation. Inoculated two-day-old seedlings of different resistant (7042R, IP19292, IP18293, IP18294, IP18295, IP18296, IP18297, IP18298 and P-310-17) and susceptible (23B, HB3, 7042S and 700651) pearl millet genotypes with S. graminicola zoospores (4 x 10⁴ zoospores/ml). The seedlings were harvested 4 hrs of post inoculation and used for the enzyme extraction. Samples were subjected to SOD activity by following the method of NBT reduction assay. 50 µg of each sample was subjected to ELISA as mentioned earlier using PAb-MnSOD at 1:20000 dilution. The reaction was read at 410 nm using microtiter plate reader. Disease incidence was also recorded in the field for the samples. Data are mean (± SD) of three independent experiments with reading determined in triplicates.