Figure 3. Immnodetection of the antibody specificity by Dot-immunobinding assay (DIBA) for:

(a) crude and pure antigen.
(b) seedlings extracts of different pearl millet genotypes
(c) root and shoot of resistant and susceptible pearl millet genotypes.

Two-day-old resistant and susceptible pearl millet seedlings were inoculated with zoospore suspension of S. graminicola (4 x 10⁴ zoospores/ml). Primary antibody was used at a dilution of 1:5000. (w, distilled water treatment; c, crude SOD extract; p, pure Mn-SOD, (s, susceptible; r, resistant; UI, uninoculated; and I, inoculated)