Figure 4. Immunoblotting for the
detection of the 35 kDa protein. Two-day-old
resistant (IP 18293) and susceptible (23B) pearl millet seedlings were
inoculated with zoospore suspension of S. graminicola (40 x 104
zoospores/ml). Seedlings harvested at 4 hrs post-inoculation were used
for the enzyme extraction. Primary antibody was used at a dilution of
1:5000. SC: Susceptible control, SI: Susceptible incoulated, RC: Resistant
control, RI: Resistant Inoculated. Band intensity was quantitated by bioprofile
image analysis ( |
|