|
|
Table 2. Determination
of PAb-Mn SOD in different tissues of pearl millet seedlings. Two-day-old
resistant (IP 18293) and susceptible (23B) pearl millet genotypes were
inoculated with S. graminicola zoospores (4 x 104
zoospores/ml). Root and shoot portions of seedlings were excised 4 hrs
of post inoculation and used for the enzyme extraction. 50 µg of each
sample was loaded to the wells of ELISA plate. Primary antibody was
used at a dilution of 1:20,000. The reaction was read at 410 nm using
microtitre plate reader. Data are mean (±SD) of three independent experiments
with reading determined in triplicates. |
|
Part of the seedling |
Absorbance at 410 nm |
|||
|
Resistant |
Susceptible |
|||
|
Uninoculated |
Inoculated |
Uninoculated |
Inoculated |
|
| Root |
0.31 ± 0.031 |
0.85 ± 0.025 |
0.37 ± 0.018 |
0.52 ± 0.024 |
| Shoot |
0.46 ± 0.034 |
0.25 ± 0.018 |
0.59 ± 0.037 |
0.57 ± 0.017 |
|
|