Figure 3. Presence of Mytilus edulis chilensis defensin-like gene in putative transgenic plants. Leaf genomic DNA was amplified by PCR using the primers described in Figure 1. The arrow indicates the expected size of the fragment (560 bp). Lanes: St, bp size standard; (-), no DNA in the amplification reaction; (+), PCR amplification of the chimeric gene-containing plasmid used to transform plant cells. N, non-transformed controls; 1-15, transgenic tobacco plant lines: lines 3, 8 and 15 were not transformed.