Figure 4. Expression of the defensin-like
transgene in transformed whole tobacco plants.
(a)
Agarose-formaldehyde gel stained with ethydium bromide.
(b) Northern
blot analysis. Arrow indicates the expected position of the hybridization
band specific for the defensin-like probe.
Total RNA from
12 independent lines (lanes 1-14) were analysed. Lanes 2, 4, and 6 – 14
were considered positive lines. NN = Control, corresponding to non transformed
plants.
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