Figure 3. PCR analyses of putative transformed IEs for the presence of CryIA(b) gene. Genomic DNA was isolated from 3-day old putative transformed IEs and amplified using the CRYF1 and CRYR1 primer combination. All 15 samples tested showed the presence of the expected 631bp, thus confirming the presence of CryIA(b) gene in putative transformed IEs. Lane 1-4: examples of positive signals detected from all 15 samples tested. M, 1 kb DNA ladder marker.