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Table 1. Characters of the selected clones from static selection and flow based selection. |
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Static Selection |
CDR3 (L) Sequence |
OD1 Mutant/ parent |
Dissoc. Rate (kd, s-1) |
Affinity constant (KD, M) |
Flow based Selection |
CDR3 (L) Sequence |
OD1 Mutant/ parent |
Dissoc. Rate (kd, s-1) |
Affinity constant (KD, M) |
| H1 (parent) |
NSRDSSGNHVV |
1 |
4.02e-3 |
2.61e-6 |
H1 (parent) |
NSRDSSGNHVV |
1 |
4.02e-3 |
2.61e-6 |
| A1 |
NSSNRPTQYVV |
1.4 |
3.79e-3 |
N.A. |
A9 |
NSSTPTQKHVV |
3.3 |
1.23e-3 |
N.A. |
| A7 |
NSSRDQEGTVV |
1.8 |
5.55e-3 |
N.A. |
A11 |
NSSPQNKTLVV |
2.5 |
2.54e-3 |
N.A. |
| C12 |
NSSKNDSVLVV |
1.6 |
1.92e-2 |
N.A. |
D7 |
NSSDQNITSVV |
3.6 |
8.65e-4 |
N.A. |
| D5 |
NSSHVILNRVV |
1.4 |
7.15e-3 |
N.A. |
D9 (H1v3) |
NSSTRHNPTVV |
4.1 |
6.05e-4 |
7.28e-8 |
| D7 |
NSSDSKNRPVV |
2.0 |
6.54e-3 |
N.A. |
D12 |
NSSQRHLPNVV |
3.9 |
7.94e-4 |
N.A. |
| E1 |
NSSQRDSLKVV |
1.7 |
3.29e-3 |
N.A. |
C1 |
NSSIPRKLIVV |
2.4 |
1.55e-3 |
N.A. |
| F11 |
NSSKTSNRDVV |
1.5 |
8.25e-3 |
N.A. |
C11 |
NSSDNGSKHVV |
2.9 |
6.72e-4 |
N.A. |
| H5 |
NSSCNQDSLVV |
1.8 |
5.36e-3 |
N.A. |
C12 |
NSSHLHNHPVV |
3.2 |
3.22e-3 |
N.A. |
| H9 (H1v2) |
NSSYCVRTLVV |
2.2 |
7.45e-3 |
6.53e-7 |
F9 |
NSSKTLNVDVV |
3.6 |
1.74e-3 |
N.A. |
| 2Average |
7.40e-3 |
1.47e-3 |
| 1OD
values represent the ratio of Mutant/parent readings, where each individual
reading is obtained by calculating OD450-OD650. |
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