Breeding

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 3, Special Issue, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile  
DOI: 10.2225/vol9-issue3-fulltext-10  
RESEARCH ARTICLE

Towards genetic transformation of local onion varieties

Pablo Marinangeli*
Departamento de Agronomía
Universidad Nacional de Sur
CERZOS (CONICET-UNSur)
Bahía Blanca, Argentina
Tel/Fax: 54 291 4862882
E-mail: pamarina@criba.edu.ar

Bernardo Deluchi
Departamento de Agronomía
Universidad Nacional de Sur
Bahía Blanca, Argentina
Tel/Fax: 54 291 4862882

Raúl Ríos
Instituto de Genética Ewald A. Favret
CICVyA, INTA
De los Reseros y Las Cabañas s/n
Castelar, Argentina
Tel/Fax: 54 11 4450-0805/1876
E-mail: rrios@cnia.inta.gov.ar

Pascual Franzone
Instituto de Genética Ewald A. Favret
CICVyA, INTA
De los Reseros y Las Cabañas s/n
Castelar, Argentina
Tel/Fax: 54 11 4450-0805/1876
E-mail: pfranzone@cnia.inta.gov.ar

Claudio Galmarini
EEA INTA La Consulta
cc. 8, La Consulta
Mendoza, Argentina
Tel/Fax: 54 2622 470304
E-mail: cgalmarini@fca.uncu.edu.ar

Fernando Rosselló
Departamento de Agronomía
Universidad Nacional de Sur
CERZOS (CONICET-UNSur)
Bahía Blanca, Argentina
Tel/Fax: 54 291 4862882
E-mail: rossello@criba.edu.ar

Néstor Curvetto
Departamento de Agronomía
Universidad Nacional de Sur
CERZOS (CONICET-UNSur)
Bahía Blanca, Argentina
Tel/Fax: 54 291 4862882
E-mail: ficurvet@criba.edu.ar

Website:  http://www.criba.edu.ar/cerzos/

*Corresponding author

Financial support: Secretaría de Ciencia y Tecnología, UNS, Agencia Nacional de Promoción Científica y Tecnológica (PICT 08-12671) and the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).

Keywords: Agrobacterium tumefaciens, Allium cepa L., callus, zygotic embryos.

Abbreviations:

ME: mature zygotic embryos
MSc: callus induction medium
MSr: regeneration medium

Abstract   Reprint (PDF)

The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of ‘Valenciana’ onion, Torrentina, Cobriza INTA and Grano de oro were in vitro cultivated for callus induction. After three to four months an average of 57.4% success for the three varieties was reached. Transformation was carried out with Agl1 or LBA 4404 A. tumefaciens strains, both carrying a binary vector containing the marker gene gus a and the selection gene nptII. Selection was done in callus induction medium containing 10 mgL-1 geneticin during three subcultures. At the end of the selection period, 342 portions of calli were recovered and transferred to regeneration medium. Of the selected calli evaluated by the expresión of the β-glucuronidase enzyme, 42% presented extensive blue areas or were completely blue. At the end of the first subculture in the regeneration medium, 54 calli were considered potentially organogenic because of the green areas observed. At the end of the wole regeneration period, just one normal plant was obtained, that was negative to PCR analysis using specific primers for gus a and nptII. All selected calli came from the Torrentina variety and the highest quantity of them were transformed with the strain LBA 4404.

 
Supported by UNESCO / MIRCEN network