Chlorinated biphenyl degradation by wild yeasts pre-cultured in biphasic systems M. Cristina Romero*
Micología Médica e Industrial, Microbiología Facultad de Ciencias Veterinarias Universidad Nacional de Av. 60 e/ 119 y 120 s/nro., 1900 La Plata, Argentina Tel: 0054 221 4247642 E-mail: reinoso@fcv.unlp.edu.ar Alejandro
Moreno Kiernan Facultad de Ciencias Agrarias y Forestales Universidad Nacional de Av. 60 e/ 119 y 120 s/nro., 1900 Tel: 0054 221 4835934 E-mail: urrutia@isis.unlp.edu.ar *Corresponding author Financial support: This work was supported by grants from the National Council of Scientific and Technological Research (CONICET) and from the National University of La Plata (UNLP- Facultad de Ciencias Veterinarias), Argentina. Keywords: biphasic system, fungal adaptation, increased solubilities, transformation rates, yeasts communities.
Environmental biotechnology
has developed as an offshoot from sanitary engineering, and only recently
the biological component of the ecosystems had been recognized as
relevant when bioremediation strategies must be chosen to solve environmental
problems. Yeasts were isolated on 2,4-dichlorobiphenyl, 2,3',4- and
2,4',5-trichlorobiphenyl, poorly soluble compounds in water, as carbon
sources. Debaryomyces castelli, Debaryomyces maramus
and Dipodascus aggregatus composed the mixed culture and represented
72% of the isolates; their degradation potential were studied in biphasic
and monophasic systems. The biphasic cultures were obtained with phenol
as the organic phase and MSM as the aqueous ones, the monophasic medium
only with MSM. Both cultures were supplied with 50, 100, 150 and 200
ppm DCB, TCB- |