Molecular Markers

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 3, Issue of April 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile  
DOI: 10.2225/vol9-issue3-fulltext-17  
RESEARCH ARTICLE

Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites

Mariana C. Pérez de la Torre
Instituto de Floricultura
INTA Castelar
Nicolás Reppetto y De los Reseros s/n. (1712)
Buenos Aires, Argentina
Tel/Fax: 54 11 4681 3736
E-mail: mpdelatorre@cnia.inta.gov.ar

Alejandro S. Escandón*
Instituto de Floricultura
INTA Castelar
Nicolás Reppetto y De los Reseros s/n. (1712)
Buenos Aires, Argentina
Tel/Fax: 54 11 4681 3736
E-mail: aescandon@cnia.inta.gov.ar  

Web site: http://www.inta.gov.ar

*Corresponding author

Financial support: Granted by INTA Project number 1837.

Keywords: ISSR, ornamentals, varietal identification.

Abbreviations:

CTAB: Cetyltrimethylammonium bromide
ISSR: Inter simple sequence repeat
MIP: Molecular identification profile
PCR: Polymerase chain reaction
Rp: Resolving power


Abstract   Reprint (PDF)

The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.

 
Supported by UNESCO / MIRCEN network 
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