Molecular Markers

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 3, Issue of April 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile  
DOI: 10.2225/vol9-issue3-fulltext-23  
RESEARCH ARTICLE

Identification of microsatellites linked to Lr47

Leonardo Sebastián Vanzetti
Grupo de Biotecnología y Rec. Genéticos
INTA EEA Marcos Juárez
Ruta 12 S/N, (2580) Marcos Juárez
Córdoba, Argentina
Tel/Fax: 54 3472 425001
E-mail: lvanzetti@mjuarez.inta.gov.ar

Juan Carlos Brevis
Department of Agronomy and Range Science
University of California
One Shields Ave. Davis, CA 95616-8515
USA
Tel: 530 752 5159
Fax: 530 752 4361
E-mail: jcbrevis@ucdavis.edu

Jorge Dubcovsky
Department of Agronomy and Range Science
University of California
One Shields Ave. Davis, CA 95616-8515
USA
Tel: 530 752 5159
Fax: 530 752 4361
E-mail: jdubcovsky@ucdavis.edu   

Marcelo Helguera*
Grupo de Biotecnología y Rec. Genéticos
INTA EEA Marcos Juárez
Ruta 12 S/N, (2580) Marcos Juárez
Córdoba, Argentina
Tel/Fax: 54 3472 425001
E-mail: mhelguera@mjuarez.inta.gov.ar


*Corresponding author

Financial support: Grants from the Government of Argentina, Proyecto Nacional INTA 52-520209 and in the US by competitive grant IFAFS 2001-04462.

Keywords: gwm 60, leaf rust, marker assisted selection, Puccinia triticina Ericks., Triticum aestivum.

Abbreviations:

PCR: polymerase chain reaction
RFLP: restriction fragment length polymorphism


Abstract   Reprint (PDF)

Leaf rust resistance gene Lr47 is located within a interstitial segment of Triticum speltoides Taush. 7S chromosome translocated to the short arm of chromosome 7A of bread wheat. This gene is resistant against currently predominant races of leaf rust from Argentina. The objectives of this study were to identify microsatellites linked to this source of resistance as a potential tool to introgress this source of resistance. Isogenic lines with and without Lr47 developed from 10 cultivars/breeding lines as well as 10 microsatellites previously mapped in 7AS chromosome were used in this study. Microsatellite gwm 60 was the only marker that co-segregated completely linked to Lr47. These data indicate that gwm 60 could be a valuable marker to introgress Lr47 in wheat germplasm.

 
Supported by UNESCO / MIRCEN network 
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