Meiotic study of Zea mays ssp. mays (2n = 40) x Tripsacum dactyloides (2 n = 72) hybrid and its progeny
María del Carmen Molina*
María Dina García
Silvia Elena Chorzempa
Financial support: FCAyF-UNLP (grant A129), FCA-UNLZ and CONICET (grant 4650).
Keywords: chromosome number, corn, eastern gamagrass, remote hybridisation.
Maize (2n = 40) x Tripsacum dactyloides (2n = 72) F1 hybrid plants (2n = 56) were obtained by embryo rescue and induction of somatic embryogenesis/organogenesis. Hybrid plants showed Tripsacum-like phenotypes, tolerance to stresses such as NaCl salinity and low temperatures. The more frequent meiotic configurations were 28II (24%), 24II + 2IV (19%) and 26II + 1IV (12%), with an average per cell of 0.55I + 25.18II + 1.19IV. Significant differences between plants were not observed. Pollen fertility ranged from 0% to 50%. After pollination with maize or Tripsacum, 20% of F1 plants have developed viable seeds, which originated the progeny. Thirty five percent of the progeny showed 2n = 56 chromosomes and F1 like-phenotypes, which suggests they have apomictic origin. The remaining plants were fertile and they showed maize-like phenotypes and different chromosome numbers (2n = 22, 24, 26, 28 and 30), because they kept the complete maize chromosome complement and some of the Tripsacum chromosomes. Meiotic cells showed pairing between chromosomes from both parental species, which suggests the possibility of genetic recombination between them.
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