Electronic Journal of Biotechnology
ISSN: 0717-3458 |
Vol. 9 No. 3, Special Issue, 2006 |
© 2006 by Pontificia Universidad Católica
de Valparaíso -- Chile |
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DOI: 10.2225/vol9-issue3-fulltext-7 |
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In
vitro culture
response of barley (Hordeum vulgare) ethylene synthesis mutant
MC 169
Carolina
Biagioli*
Instituto
de Genética “Ewald A. Favret” (IGEAF)
CICVyA, INTA. C.C. 25 (1712) Castelar
Buenos Aires, Argentina
Tel/Fax: 011 4450 0805
E-mail: cbiagioli@cnia.inta.gov.ar
Raúl Rios
Instituto
de Genética “Ewald A. Favret” (IGEAF)
CICVyA, INTA. C.C. 25 (1712) Castelar
Buenos Aires, Argentina
Tel/Fax: 011 4450 0805
E-mail: rrios@cnia.inta.gov.ar
Alicia Basso
Facultad
de Agronomía
Universidad de Buenos Aires
Av. San Martín 4453
Buenos Aires, Argentina
Tel/Fax: (011) 4524-8000
E-mail: abasso@agro.uba.ar
Pascual Franzone
Instituto
de Genética “Ewald A. Favret” (IGEAF)
CICVyA, INTA. C.C. 25 (1712) Castelar
Buenos Aires, Argentina
Tel/Fax: 011 4450 0805
E-mail: pfranzone@cnia.inta.gov.ar
Alberto Prina
Instituto
de Genética “Ewald A. Favret” (IGEAF)
CICVyA, INTA. C.C. 25 (1712) Castelar
Buenos Aires, Argentina
Tel/Fax: 011 4450 0805
E-mail: aprina@cnia.inta.gov.ar
*Corresponding
author
Keywords:
callus induction, ethylene, habituated calli, hydroponic culture,
regeneration.
Abbreviations: |
2,4-D: 2,4 dichlorophenoxyacetic acid
AgNO3: silver nitrate
ANA: α-naphthaleneacetic-acid
AVG: aminoethoxivinilglicine
BAP: 6-benzylaminopurine
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Although it is generally
accepted that plant in vitro culture response is influenced
by the donor genotype, the genetic and molecular bases of this phenomenon
are barely known. As a consequence, the optimization of tissue culture
protocols is mainly empirically done. Researchers of the IGEAF studied
the genetic basis of the in vitro regeneration of various
plant species, including the tissue culture response of artificially
induced barley mutants. One barley mutant, MC 169, carries a nuclear
gene, recently described controlling the root growth in hydroponic
cultivation. Under this condition, the roots of MC 169 mutant plants
were longer than those of the original wild type line MC 182,
a fact that was associated with a reduced ethylene
biosynthesis. On the other hand, it is known that ethylene accumulation
is inhibitory for in vitro regeneration of several plant species.
In this study, we compared the in vitro culture response of
mutant MC 169 with that of its mother line MC 182. The data about
induction and regeneration of calli as well as those of habituated
calli formation demonstrated that mutant MC 169 and its mother line
MC 182 show a similar in vitro behaviour.
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