Electronic Journal of Biotechnology
ISSN: 0717-3458 |
Vol. 9 No. 3, Special Issue, 2006 |
© 2006 by Pontificia Universidad Católica
de Valparaíso -- Chile |
|
DOI: 10.2225/vol9-issue3-fulltext- |
|
RAPD and freezing resistance in
Eucalyptus globulus
Marta Fernández R.
Centro
de Biotecnología
Universidad de Concepción
Barrio Universitario s/n, Concepción, Chile
Tel: 056 41 207299
Fax: 056 41 247517
E-mail: martfern@udec.cl
Sofía
Valenzuela A.*
Centro de Biotecnología
Facultad de Ciencias Forestales
Universidad de Concepción
Barrio Universitario s/n, Concepción, Chile
Tel: 056 41 204697
Fax: 056 41 247517
E-mail: sofvalen@udec.cl
Claudio
Balocchi L.
Bioforest S.A.
Camino a Coronel Km15, Concepción, Chile
Tel: 056 41-390438
Fax: 056 41 390439
E-mail: cbalocch@arauco.cl
*Corresponding
author
Financial support:
INNOVA Biobio Grant 03 B1 210 L1.
Keywords:
Eucalyptus globulus, freezing resistance, molecular markers,
RAPD.
Eucalyptus globulus
is the second most important forest species in Chile,
after Pinus radiata. The main advantages of E. globulus
are its fast growth (25 m3/ha/year) and its excellent wood
quality for kraft pulp production. On the negative side, its low freezing
tolerance has been an obstacle for the expansion of plantations,
specifically on the foothills of the Andes.
The difference in the freezing resistance between clones of E.
globulus has a genetic base and, therefore, it could be detected
through DNA molecular markers. Fifteen clones of E. globulus,
eight classified as freezing resistant and seven as freezing sensitive
were analyzed using the technique of RAPD, in order to obtain molecular
markers that could differentiate between freezing sensitive and resistant
clones. Eighteen primers amplified reproducible bands. Three
bands were only present in freezing resistant clones, two bands of
768 bp, 602 bp obtained with UBC 218 primer and one band of 248 bp
obtained with UBC 237 primer. The preliminary results indicate that
polymorphism can be observed with the primers employed, but it is
not possible to associate the bands with the cold resistance or susceptibility
in E. globulus.
|