Table 1. Callus induction, transformation,
selection and plantlet regeneration from mature embryos of three onion
varieties using Agl1 GI and LBA 4404 GI Agrobacterium tumefaciens
strains as transfection vectors. |
Onion Varieties |
Cultivated ME |
Callus induction (%) |
Transformed calli |
Selected callus pieces |
Calli with green areas |
Regenerated plants |
||||||||
Agl1 |
LBA |
Total |
Agl1 |
LBA |
Total |
Agl1 |
LBA |
Total |
Agl1 |
LBA |
Total |
|||
Torrentina |
662 |
37.0 |
136 |
105 |
241 |
73 |
269 |
342 |
13 |
41 |
54 |
0 |
1 |
1 |
Cobriza |
178 |
88.7 |
64 |
51 |
115 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
G. de Oro |
210 |
95.2 |
85 |
91 |
176 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Total |
1050 |
57.4 |
285 |
247 |
532 |
73 |
269 |
342 |
13 |
41 |
54 |
0 |
1 |
1 |
|