Basal Medium

Auxin/
Giberellin

Citokynine

Carbon source

Other additives

Callus Induction Medium (CIM)

MS/2

NAA 3 mg/L

GA₃ 5 mg/L

BAP 1 mg/L

3% sucrose

Embryogenesis Induction Medium MIE

MS full strength

BAP 10 mg/L

6% glucose

Adenina 40 mg/L

Putrescine 161.1 mg/L

Embryos Germination Medium (EGM)

MS full strength

GA₃ 0.01mg/L

BAP 0.2 mg/l

3% sucrose

Putrescine 161.1 mg/L

Secondary Embryogenesis Medium (SEM)

MS full strength

BAP 1 mg/L

2% sucrose

Casein hydrolyzed 200 mg/L

Putrescine 161.1 mg/L Adenine 40 mg/L