Figure 1. Purification of Taq polimerase and activity assay.  
(a) SDS-PAGE (12.5%) of aliquots of the preparation at different purification steps. Lane 1: solubilized proteins after 11 hrs of IPTG (1 mM) induction. Lane 2: SN obtained after the sonication step. Lane 3: proteins remaining after purification by heat.
(b) SDS-PAGE (12.5%, silver staining) of the commercial (lane 1) and produced (lane 2) Taq polimerase.
(c) Amplification products of the AT2 Ang II receptor, obtained with the produced Taq polimerase (lanes 1-8) and commercial one (lanes 9-10). Lanes 1-8: volumes of Taq employed (µl): 1 (0.3), 2 (0.4), 3 (0.5), 4 (0.6), 5 (0.7), 6 (0.8), 7 (0.9), 8 (1). Lanes 9-10: 0.3 and 0.4 µl, commercial Taq. MW: molecular weight ladder, 1 kb.


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