Figure 2. Effect of metals on protein oxidation. (a) Total carbonyl groups content (C = 0.83 µmol COOH mg-1 protein). (b) Extracts derivatized with 2,4 DNPH were separated by SDS-PAGE in 12% gel and transferred onto nitrocellulose membranes. Derivatized proteins were detected with antibody anti-DNP, bands were visualized as described Material and Methods and photographed with a Fotodyn. Analysis of the image was performed with GelPro software. The positions of molecular mass markers (in kilodaltons) are shown on the left. The electrophoresis and western blot data shown are representative of several experiments.