|
|
Table 1. Effect of selective proteinase inhibitors
and activators on protease activity. Characterization of proteolytic activities
at pH 5.2. |
|
Compound |
Final concentration |
Remaining activity (%) |
|
|
None (control) |
|
100 |
|
|
Methanol (15 µl) |
|
103 |
|
|
Inhibitors |
PMSF |
100 µM |
86 |
|
AEBSF |
100 µM |
86 |
|
|
IAA |
500 µM |
39 |
|
|
E-64 |
5 µM |
25 |
|
|
Pepstain |
20 µM |
63 |
|
|
EDTA |
1 mM |
74 |
|
|
o-phenanthroline |
1 mM |
17 |
|
|
Bestain |
50 µM |
78 |
|
|
Activators |
DTT |
4 mM |
118 |
|
2-mercaptoethanol |
2.5 mM |
120 |
|
|
Cysteine |
4 mM |
118 |
|
|
|