Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002
Moura de Luna
Galba Maria de
support: The work was financed by
Keywords: biosurfactant, Candida, emulsifier, fermentation, glucose, vegetal oil.
Evaluation of both
tenso-active and emulsifying activities indicated that a biosurfactant
was produced by the newly isolated and promising strain Candida
glabrata isolated from mangrove sediments. The extracellular water-soluble
emulsifying agent was isolated and identified as a heteropolymer.
The maximum of bioemulsifier production was observed when the strain
was grown on soluble and insoluble substrates cotton seed oil plus
glucose, reaching values of 10.0 g/l after 144 hrs at 200 rpm. The
cell-free culture broth containing the examined agent lowered the
surface tension of the medium to 31 mN/m. Stable and compact emulsions
with emulsifying activity of 75% of cotton seed oil were detected.
The emulsification capacity remained practically unaltered within
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