Abstract - Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002

Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002

Leonie Asfora Sarubbo
Núcleo de Pesquisas em Ciências Ambientais (NPCIAMB)
Departamento de Química
Universidade Católica de Pernambuco
Rua Nunes Machado, n.42, Bl J, Boa Vista
CEP: 50050-590
Recife-PE, Brazil
Tel: 55 81 21194017
Fax: 55 81 21194043
E-mail: leonie@unicap.br

Juliana Moura de Luna
Núcleo de Pesquisas em Ciências Ambientais (NPCIAMB)
Mestrado em Ciências Biológicas
Centro de Ciências Biológicas
Universidade Federal de Pernambuco
Av, Prof. Moraes Rêgo, s/n, Cidade Universitária
CEP: 50900-000
Recife-PE, Brazil

Galba Maria de Campos-Takaki*
Núcleo de Pesquisas em Ciências Ambientais (NPCIAMB)
Departamento de Química
Universidade Católica de Pernambuco
Rua Nunes Machado, n.42, Bl J, Boa Vista
CEP: 50050-590
Recife-PE, Brazil
Tel: 55 81 21194017
Fax: 55 81 21194043
E-mail: takaki@unicap.br

Website: http://www.unicap.br

*Corresponding author

Financial support: The work was financed by Agência Financiadora de Estudos e Projetos (FINEP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Universidade Católica de Pernambuco (UNICAP), Pernambuco, Brazil.

Keywords: biosurfactant, Candida, emulsifier, fermentation, glucose, vegetal oil.

Evaluation of both tenso-active and emulsifying activities indicated that a biosurfactant was produced by the newly isolated and promising strain Candida glabrata isolated from mangrove sediments. The extracellular water-soluble emulsifying agent was isolated and identified as a heteropolymer. The maximum of bioemulsifier production was observed when the strain was grown on soluble and insoluble substrates cotton seed oil plus glucose, reaching values of 10.0 g/l after 144 hrs at 200 rpm. The cell-free culture broth containing the examined agent lowered the surface tension of the medium to 31 mN/m. Stable and compact emulsions with emulsifying activity of 75% of cotton seed oil were detected. The emulsification capacity remained practically unaltered within a wide pH (2-12), temperature (4-80ºC) ranges and under NaCl concentrations up to 10%.