Production of Rhodotorula glutinis: a yeast that secretes α-L-arabinofuranosidase
Financial support: This investigation
was financed by the grant Fondef D98I1037,
Keywords: α-L-arabinofuranosidase yeast, enzymes, wine.
Rhodotorula glutinis is a yeast that secretes the enzyme α-L-arabinofuranosidase (E.C. 220.127.116.11) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.
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