Microbial Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 4, Issue of July 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile Received October 20, 2005 / Accepted March 13, 2006
DOI: 10.2225/vol9-issue4-fulltext-9  

Cotton genetic diversity study by AFLP markers

Naveed Murtaza#
Department of Plant Breeding and Genetics
Bahauddin Zakaryia University
Multan, Pakistan
E-mail: naveedm@bzu.edu.pk

Financial support: Japan Society for the Promotion of Science.

Keywords: AFLP, cluster analysis, cotton, genetic diversity, PCR.

#Present address: National Food Research Institute, Kannondai, Tsukuba, Ibaraki, 305-0856, Japan. Tel: 81 9092335584; Fax: 81 298387321.


AFLP: amplified fragment length polymorphism
PCR: polymerase chain reaction

Full Text

Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertainthe intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. Theeffectiveness of this method to distinguish inter and intra specificdifference in cotton could be handy in cultivar recognition and in marker assistedparental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The AFLP data assigned the genotypesinto groups that corresponded with their origin and lineagerelationships and showed a narrow genetic base among these cultivars.

Supported by UNESCO / MIRCEN network
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