Figure 2. cDNA Specific Primer Pairs for PCR Screen of λgt10 Library. Primer pairs were designed to identify two different cytoplasmic myosin isoforms XlMyo7a and XlMyo1d from short cloned regions in an isoform specific region of the myosin head region. Primer pairs were tested against four templates, XlMyo1c, 7a and 1d, and lambda DNA, using the reaction conditions described in the Materials and Methods. The panel on the left shows products amplified using the 7a primer pair. The panel on the right shows similar results for 1d primer pair.