Figure 4. Influence of ultra sonication with pectinase enzyme treatment on transformation frequency. Legends: 1: Control (No Agrobacterium rhizogenes infection); 2: Manual wounding prior to co cultivation (A); 3: Sonication treatment prior to co cultivation (B); 4: Sonication with 0.1% pectinase enzyme treatment prior to co cultivation (C1); 5: Sonication with 0.5% pectinase enzyme treatment prior to co cultivation (C2); 6-Sonication with 1% pectinase enzyme treatment prior to co cultivation (C3).