Figure 8. Influence of ultra sonication with acetosyringone treatment on transformation frequency. Legends: 1: Control (No Agrobacterium rhizogenes infection); 2: Manual wounding prior to co cultivation (A); 3: Sonication treatment prior to co cultivation (B); 4: Sonication with 50 µM acetosyringone in the co cultivation medium (F1); 5: Sonication with 100 µM acetosyringone in the co cultivation medium (F2); 6: Sonication with 150 µM acetosyringone in the co cultivation medium (F3).