Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L.
Sreedhar Reddampalli Venkataramareddy
Ravishankar Gokare Aswathanarayana
support: Senior and Junior Research Fellowship
from the Council of Scientific and Industrial
Keywords: Aspergillus, calcium, culture filtrate, dried cell powder, elicitor, glutathione, methyljasmonate, Rhizophus, thidiazuron.
Genetically transformed roots
of red beet produce copious levels of peroxidase (POD) - a multifunctional
enzyme with a number of commercial applications. In an effort to elicit
the POD activity, the cultures were treated with biotic elicitors
such as dry cell powders of microbial cultures (0.1-0.5% w/v) and
the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors,
particularly metal ions (2-8 folds of that present in the nutrient
medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules
such as Glutathione (at 0.5-
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