Modification of the activity of an α-amylase from Bacillus licheniformis by several surfactants
Vicente Bravo Rodríguez*
Encarnación Jurado Alameda
Juan Francisco Martínez Gallegos
Antonia Reyes Requena
Ana Isabel García López
Joaquim Manuel Sampaio Cabral
Luis Joaquim Pina da Fonseca
Financial support: Fellowship awarded to Juan Francisco Martínez Gallegos by Spanish Ministry of Science and Education, FPU fellowships program.
Keywords: α-amylase, alkyl polyglycosides, enzymatic activity, fatty alcohol ethoxylates, linear alkyl benzene sulfonate, nonyl phenol ethoxylate.
The influence of different commercial surfactants on the enzymatic activity of a commercial α-amylase from Bacillus licheniformis (Termamyl 300 L) has been studied. As non-ionic surfactants, alkyl polyglycosides (Glucopon® 215, Glucopon® 600 and Glucopon® 650) were studied, as were fatty alcohol ethoxylates (Findet 1214N/23 and Findet 10/15), and nonyl phenol ethoxylate (Findet 9Q/21.5NF). Also, an anionic surfactant, linear alkyl benzene sulfonate (LAS) was assayed. In general, none of the non-ionic surfactants studied, except Findet 10/15, vary substantially the enzymatic activity. Findet 10/15 has the strongest hydrophobic character and reduces the enzymatic activity more significantly the greater its concentration. Regarding LAS, this surfactant significantly depressed enzymatic activity, presumably due to the electrostatic interactions caused by its anionic character.
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