Microbial Biotechnology

Biotechnology of Human Disorders
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 9 No. 5, Issue of October 15, 2006
© 2006 by Pontificia Universidad Católica de Valparaíso -- Chile Received November 15, 2005 / Accepted March 22, 2006
DOI: 10.2225/vol9-issue5-fulltext-2  
RESEARCH ARTICLE

Designing and validation of genus-specific primers for human gut flora study 

Rani Rekha
 School of Life Sciences
Jawaharlal Nehru University
New Delhi 110067
India
Tel: 91 011 26704516
Fax: 91 011 26717580
E-mail: rekhs2004garg@gmail.com

Moshahid Alam Rizvi
 Department of Biosciences
Jamia Millia Islamia
New Delhi 110025
India
E-mail: rizvi_ma@yahoo.com

Paul Jaishree*
 School of Life Sciences
Jawaharlal Nehru University
New Delhi 110067
India
Tel: 91 011 26704516
Fax: 91 011 26717580
E-mail: jpaul33@hotmail.com

*Corresponding author

Financial support: University Grant Commission, New Delhi, India.

Keywords: 16S rRNA based primers, anaerobic bacteria; diarrhoea patient, polymerase chain reaction.

Abbreviations:

AAD: Antibiotic Associated Diarrhoea
BLAST: Basic Local Alignment Search Tool
IMTECH: Institute of Microbial Technology
MTCC: Microbial Type Culture Collection Center
PCR: polymerase chain reaction
RFLP: Restriction Fragment Length Polymorphism
rRNA: ribosomal ribonucleic acid
U: Units
Abstract
Full Text

The aim of this study, was to design and validate 16S rRNA targeted oligonucleotide genus specific primers for amplifying the predominant members of gut flora using polymerase chain reaction. Primers were validated against human faecal samples. gut flora of a normal individual was compared with that of two diseased individuals. Our observations showed that the genera Lactobacillus, Bacteroides, Peptococcus, Bifidobacterium, and E. coli were invariably present in all studied subjects however, the absence of butyrate producing bacteria Ruminococcus and Peptostreptococcus were significant. Presence of the members of the genus, Campylobacter in both the diseased samples were also unusual.
Supported by UNESCO / MIRCEN network
Home | Mail to Editor | Search | Archive