Application of response surface methodology for glucosyltransferase production and conversion of sucrose into isomaltulose using free Erwinia sp. cells
Haroldo Yukio Kawaguti*
Hélia Harumi Sato
Financial support: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Financiadora de Estudos e Projetos em Pesquisa (FINEP) and GETEC Guanabara Química Industrial S.A.
Keywords: batch process, Erwinia sp., free cells, glucosyltransferase, isomaltulose, response surface methodology.
Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. The Experimental design and response surface methodology were applied for the optimization of the nutrient concentration in the culture medium for the enzyme production in shaken flasks at 200 rpm and
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