Table 3. Elicitation of POD activity (x 106 U L-1) purified biotic (GSH and Mej) and abiotic elicitors (Mg, Ca and TDZ). The values are mean ± SE of six replicates.


Treatment
Time of elicitor addition (day)

15

18

20

Control*

 

2.75 ± 0.05

2.85 ± 0.03

2.80 ± 0.04

Glutathione (GSH)

2 mM
4 mM
6 mM
8 mM
10 mM

9.46 ± 0.32a
4.81 ± 0.23ef
4.44 ± 0.22f
4.22 ± 0.18gf
4.73 ± 0.09ef

5.65 ± 0.41b
6.08 ± 0.07ab
5.63 ± 0.21b
6.59 ± 0.38a
6.29 ± 0.12a

5.73 ± 0.32cd
5.84 ± 0.21cd
7.18 ± 0.10cb
6.24 ± 0.41cd
6.63 ± 0.12c

Methyl jasmonate (Mej)

20 µM
40 µM
60 µM
80 µM
100 µm

1.39 ± 0.10i
1.83 ± 0.41ih
1.59 ± 0.05i
1.15 ± 0.23ji
1.17 ± 0.08ji

2.33 ± 0.38ef
1.66 ± 0.15f
1.64 ± 0.03f
1.71 ± 0.21ef
1.68 ± 0.08ef

1.38 ± 0.23h
1.52 ± 0.12h
1.43 ± 0.12h
0.11 ± 0.05ih
1.39 ± 0.06h

Magnesium (Mg)

2 fold
4 fold
6 fold

6.10 ± 0.23ed
6.20 ± 0.10ed
7.40 ± 0.23c

5.01 ± 0.31cb
5.04 ± 0.05cb
5.51 ± 0.21b

6.05 ± 0.12cd
7.20 ± 0.31cb
8.49 ± 0.23a

Calcium (Ca)

2 fold
4 fold
6 fold

7.15 ± 0.41cd
6.67 ± 0.21d
5.87 ± 0.10ed

6.38 ± 0.05a
6.39 ± 0.12a
5.26 ± 0.08cb

6.18 ± 0.41cd
7.87 ± 0.21ab
7.68 ± 0.23b

Thidiazuron (TDZ)

0.25 ppm
0.50 ppm
1.00 ppm

5.54 ± 0.23e
5.54 ± 0.22e
5.04 ± 0.04ef

3.75 ± 0.23cd
3.61 ± 0.10d
6.85 ± 0.23a

6.74 ± 0.12c
3.53 ± 0.34f
3.63 ± 0.23f

*Though simultaneous controls for all the treatments were used, control data of all the experiments were averaged to minimize error.
Mean ± SE followed by the same letter within the column are not statistically significant at p ≤ 0.05 according to ANOVA.


Supported by UNESCO / MIRCEN network